Genetic studies on cycloheximide-resistant strains of Schizosaccharomyces pombe

Extending the Schizosaccharomyces pombe Molecular Genetic Toolbox

Targeted alteration of the genome lies at the heart of the exploitation of S. pombe as a model system. The rate of analysis is often determined by the efficiency with which a target locus can be manipulated. For most loci this is not a problem, however for some loci, such as fin1+, rates of gene targeting below 5% can limit the scope and scale of manipulations that are feasible within a reasona...

[Schizosaccharomyces pombe].

Effect of 2-deoxyglucose on Schizosaccharomyces pombe.

Megnet, Roland (Institut für Allgemeine Mikrobiologie der Universität, Bern, Switzerland). Effect of 2-deoxyglucose on Schizosaccharomyces pombe. J. Bacteriol. 90:1032-1035. 1965.-Cultivation of Schizosaccaromyces pombe in a medium containing 2-deoxyglucose (100 mug/ml) results in the death of the cells after an initial period of apparently normal growth. At higher deoxyglucose concentration (4...

Corrigendum: Site Specific Genetic Incorporation of Azidophenylalanine in Schizosaccharomyces pombe.

“We express our sincere gratitude to the WPH Proteomics Research Technology Platform for the contribution to the mass spectrometry analysis. In addition, we would like to express our thanks to MKB laboratory members for discussion. This work was supported by research funds from National University of Singapore, Wellcome Trust (WT101885MA), The Royal Society, Temasek Life Sciences Laboratory and...

Site Specific Genetic Incorporation of Azidophenylalanine in Schizosaccharomyces pombe

The diversity of protein functions is impacted in significant part by the chemical properties of the twenty amino acids, which are used as building blocks for nearly all proteins. The ability to incorporate unnatural amino acids (UAA) into proteins in a site specific manner can vastly expand the repertoire of protein functions and also allows detailed analysis of protein function. In recent yea...